Dermal peptide delivery using enhancer molecules and colloidal carrier systems - part V#: Influence of enhancers on the permeation of PKEK through snake skin.

ABSTRACT

In a previous study, it was shown, that shed snake skin is a good alternative model membrane for the human stratum corneum (SC). In this study, the influence of the enhancers dimethyl sulfoxide (DMSO), 1,2-propanediol, 1,3-butanediol, 1,2-pentanediol, 1,2-hexanediol and 1,2-octanediol in a concentration of 10 % on the permeation of l-prolyl- l-lysyl-l-α-glutamyl-l-lysin (PKEK) through shed snake skin was conducted. Pharmacokinetic parameters (diffusion coefficient, permeation coefficient, t-lag, Flux) were calculated. All examinations were performed on the skin of an individual and thus allowed a very good comparability of the data. All enhancers have overcome the shed snake skin and could be proven in the acceptor. DMSO does not affect the permeability of the membrane. Nevertheless, PKEK permeates faster in the presence of DMSO than PKEK being used alone. PKEK permeated the same, no matter if an auxiliary material was added or not. Without their addition, in all other enhancers no significant difference towards permeation could be determined.