Single-Cell DNA-Seq and RNA-Seq in Cancer Using the C1 System.
Adv Exp Med Biol
; 1129: 27-50, 2019.
Article
em En
| MEDLINE
| ID: mdl-30968359
ABSTRACT
Heterogeneous phenotypes of cancer cells enable them to adapt to various environments. The heterogeneity results from diversity of genome, transcriptome, and epigenome at a single-cell level. The C1 system can automatically perform single-cell capture and whole genome amplification (WGA) or whole transcription amplification (WTA) by MDA or Smart-Seq, respectively. Here, we describe the protocols for WGA and WTA from a single cell by using the C1 system and the protocols for sequence library preparation from amplified gDNA and cDNA. We also described about the computational analysis for single-cell data of cancer.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise de Sequência de RNA
/
Análise de Sequência de DNA
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Técnicas de Amplificação de Ácido Nucleico
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Análise de Célula Única
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Neoplasias
Limite:
Humans
Idioma:
En
Revista:
Adv Exp Med Biol
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Japão