A chromosome-scale genome assembly and dense genetic map for Xenopus tropicalis.
Dev Biol
; 452(1): 8-20, 2019 08 01.
Article
em En
| MEDLINE
| ID: mdl-30980799
The Western clawed frog Xenopus tropicalis is a diploid model system for both frog genetics and developmental biology, complementary to the paleotetraploid X. laevis. Here we report a chromosome-scale assembly of the X. tropicalis genome, improving the previously published draft genome assembly through the use of new assembly algorithms, additional sequence data, and the addition of a dense genetic map. The improved genome enables the mapping of specific traits (e.g., the sex locus or Mendelian mutants) and the characterization of chromosome-scale synteny with other tetrapods. We also report an improved annotation of the genome that integrates deep transcriptome sequence from diverse tissues and stages. The exon-intron structures of these genes are highly conserved relative to both X. laevis and human, as are chromosomal linkages ("synteny") and local gene order. A network analysis of developmental gene expression will aid future studies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mapeamento Cromossômico
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Cromossomos
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Genoma
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Perfilação da Expressão Gênica
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Anotação de Sequência Molecular
Limite:
Animals
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Humans
Idioma:
En
Revista:
Dev Biol
Ano de publicação:
2019
Tipo de documento:
Article
País de publicação:
Estados Unidos