The endocytic pathways of carbon dots in human adenoid cystic carcinoma cells.
Cell Prolif
; 52(3): e12586, 2019 May.
Article
em En
| MEDLINE
| ID: mdl-30997713
ABSTRACT
OBJECTIVES:
This study aimed at investigating cellular uptake pathways of carbon dots (CDs) in human adenoid cystic carcinoma cell line ACC-2. MATERIALS ANDMETHODS:
We synthesized CDs using a hydrothermal method with citric acid and polyethylenimine (PEI, Mw = 25 000). The CDs incubated with the ACC-2 cells showed their bioimaging capabilities using a confocal microscopy test. Flow cytometry was used to analyse cellular uptake pathways of CDs in ACC-2 cells.RESULTS:
Our findings indicated that CDs possessed good biocompatibility in ACC-2 cells. CDs were endocytosed mainly via micropinocytosis and energy-dependent pathways.CONCLUSIONS:
In general, these findings suggested that CDs had excellent biomedical imaging properties for ACC-2 cells and there was a potential opportunity to develop biomedical applications.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias das Glândulas Salivares
/
Carbono
/
Carcinoma Adenoide Cístico
/
Endocitose
/
Nanopartículas
Limite:
Humans
Idioma:
En
Revista:
Cell Prolif
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China