A novel Ca2+-binding protein influences photosynthetic electron transport in Anabaena sp. PCC 7120.
Biochim Biophys Acta Bioenerg
; 1860(6): 519-532, 2019 06 01.
Article
em En
| MEDLINE
| ID: mdl-31034800
ABSTRACT
Ca2+ is a potent signalling molecule that regulates many cellular processes. In cyanobacteria, Ca2+ has been linked to cell growth, stress response and photosynthesis, and to the development of specialist heterocyst cells in certain nitrogen-fixing species. Despite this, the pathways of Ca2+ signal transduction in cyanobacteria are poorly understood, and very few protein components are known. The current study describes a previously unreported Ca2+-binding protein which was called the Ca2+ Sensor EF-hand (CSE), which is conserved in filamentous, nitrogen-fixing cyanobacteria. CSE is shown to bind Ca2+, which induces a conformational change in the protein structure. Poor growth of a strain of Anabaena sp. PCC 7120 overexpressing CSE was attributed to diminished photosynthetic performance. Transcriptomics, biophysics and proteomics analyses revealed modifications in the light-harvesting phycobilisome and photosynthetic reaction centre protein complexes.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fotossíntese
/
Proteínas de Bactérias
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Cálcio
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Anabaena
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Transporte de Elétrons
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Biochim Biophys Acta Bioenerg
Ano de publicação:
2019
Tipo de documento:
Article