Th2/Th17 cytokine profile in phenotyped Greek asthmatics and relationship to biomarkers of inflammation.
Respir Med
; 151: 102-110, 2019 05.
Article
em En
| MEDLINE
| ID: mdl-31047104
ABSTRACT
OBJECTIVE:
The aim of the present study was to investigate the Th2/Th17 pathway in asthmatic patients and also the relationship to asthma severity and biomarkers of inflammation.METHODS:
90 asthmatic patients, 51 patients with severe, 39 patients with mild asthma and 98 healthy controls were included. Skin prick tests, blood eosinophils, total serum IgE and exhaled FeNO were evaluated. Serum levels of IL-4, IL-5, IL-13, IL-6, IL-17A, IL-23 and TGFß1 were determined by Flow Cytometry using a panel kit (AimPlex Biosciences). The SNP of IL17A (rs17880588) was genotyped using reverse transcriptase polymerase chain reaction (RT-PCR).RESULTS:
The genotype of the SNP in IL17A (rs 17880588) was similar among all three groups. Serum levels of IL-4, IL-5, IL-13, IL-6, IL-17A and IL-23 were higher in asthmatics compared to controls (pâ¯<â¯0.05). In addition, IL-17A and IL-4 serum levels were found significantly elevated in patients with allergic asthma (pâ¯<â¯0.05). Furthermore, IL-4, IL-5, IL-13 and IL-23 were found significantly higher in patients with eosinophil cut off values above 300â¯cells/µl (pâ¯<â¯0.05). IL-17A levels were positively correlated with FeNO values in severe asthmatics with eosinophils>400â¯cells/µl.CONCLUSIONS:
The above findings suggest the coexistence of Th2/Th17 pathway in severe, eosinophilic and in allergic asthma.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Asma
/
Interleucinas
/
Células Th2
/
Fator de Crescimento Transformador beta1
/
Células Th17
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
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Male
/
Middle aged
País/Região como assunto:
Europa
Idioma:
En
Revista:
Respir Med
Ano de publicação:
2019
Tipo de documento:
Article
País de publicação:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
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UK
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UNITED KINGDOM