Antigen presentation in brain: MHC induction on brain endothelium and astrocytes compared.
Immunology
; 60(3): 453-9, 1987 Mar.
Article
em En
| MEDLINE
| ID: mdl-3106198
ABSTRACT
Primary cultures of rat brain endothelium and astrocytes were cultured in vitro, stimulated with interferon-gamma (IFN gamma), and the levels of MHC expression were then measured by an enzyme immunoassay (EIA). Class I expression is enhanced on brain endothelium by Day 1 following stimulation, and attains a plateau level of expression. Class II is normally absent, but starts to appear at Day 2, and continues to increase until Day 5. Class II rat I-A homologue is induced much more strongly than I-E, and the dose-response curves show that I-A expression is dependent on interferon dose within the range 2-500 units/ml, whereas class I enhancement is uniform over this range. The endothelium was compared with astrocytes, in regard to MHC induction. The surface density of class I and class II molecules was lower on the astrocytes in all conditions, and both class I enhancement and class II induction were in some cases slower to appear than on endothelium treated similarly. We also describe a pre-astrocytic cell line, C9, which shows strong I-E expression when stimulated with IFN gamma, but is apparently unable to express I-A. The implications of these findings for the development of immune reactions in the brain are discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Astrócitos
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Complexo Principal de Histocompatibilidade
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Células Apresentadoras de Antígenos
Limite:
Animals
Idioma:
En
Revista:
Immunology
Ano de publicação:
1987
Tipo de documento:
Article