Adsorption of DNA by using polydopamine modified magnetic nanoparticles based on solid-phase extraction.
Anal Biochem
; 579: 9-17, 2019 08 15.
Article
em En
| MEDLINE
| ID: mdl-31078490
ABSTRACT
A polydopamine magnetic composite (PDA@Fe3O4) was prepared for the extraction of human genomic DNA and characterized by transmission electron microscopy, X-ray diffraction, FT-IR spectrometer, zeta potential and vibrating sample magnetometry. PDA@Fe3O4 based on magnetic solid phase extraction (MSPE) method have highly efficient capture of genomic deoxyribonucleic acid (DNA)and gene fragments ranging from about 100 bp to 200 bp. Compared with commercial beads (Shenggong, China) and spin column nucleic acid extraction kit (Tiangen, China), the PDA coated magnetic nanoparticles display superior genomic DNA extraction capacity (116â¯mg/g) and yield (90.2%). The isolation protocol used the solutions (composed of PEG and NaCl) with a specific pH for the binding and release of DNA. The procedure can be attributed to the charge switch of amino and hydroxyl groups on surface of the magnetic particle. The extracted DNA with high quality (A260/A280â¯=â¯1.82⯱â¯0.04) can be directly used as template for polymerase chain reaction (PCR) followed by agarose gel electrophoresis. The results showed the new composite to be an ideal adsorbent for separation of DNA which had the advantage of its low cost, high extraction capacity and yield.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
DNA
/
Extração em Fase Sólida
/
Nanopartículas de Magnetita
/
Indóis
Limite:
Humans
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China