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Extracellular transglycosylase and glyceraldehyde-3-phosphate dehydrogenase attributed to the anti-staphylococcal activity of Lactobacillus plantarum USM8613.
Ong, Jia Sin; Taylor, Todd D; Wong, Chyn Boon; Khoo, Boon Yin; Sasidharan, Sreenivasan; Choi, Sy Bing; Ohno, Hiroshi; Liong, Min Tze.
Afiliação
  • Ong JS; School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia.
  • Taylor TD; Laboratory for Microbiome Sciences, RIKEN Center for Integrative Medical Sciences, Kanagawa, 230-0045, Japan; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800, Penang, Malaysia.
  • Wong CB; School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia.
  • Khoo BY; Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia.
  • Sasidharan S; Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia.
  • Choi SB; School of Data Sciences, Perdana University, 43400, Selangor, Malaysia.
  • Ohno H; Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, Kanagawa, 230-0045, Japan; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800, Penang, Malaysia. Electronic address: hiroshi.ohno@riken.jp.
  • Liong MT; School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800, Penang, Malaysia. Electronic address: mintze.liong@usm.my.
J Biotechnol ; 300: 20-31, 2019 Jul 20.
Article em En | MEDLINE | ID: mdl-31095980
Increasing levels of antibiotic resistance in pathogens, including Staphylococcus aureus, remains a serious problem for public health, leading to the need for better alternative antimicrobial strategies. The antimicrobial proteins produced by Lactobacillus plantarum USM8613 attributed to its anti-staphylococcal activity were identified as extracellular transglycosylase and glyceraldehyde-3-phosphate dehydrogenase (GADPH), both with different mechanisms of action. Extracellular transglycosylase, which contains a LysM domain, exerts a cell wall-mediated killing mechanism, while GADPH penetrates into S. aureus cells and subsequently induces the overexpression of autolysis regulators, resulting in S. aureus autolysis. Both extracellular transglycosylase and GADPH exert anti-inflammatory effects in S. aureus-infected HaCaT cells by reducing the expression and production of TLR-2, hBDs and various pro-inflammatory cytokines (IL-1α, IL-1ß, IL-6, TNF-α, and IL-8). Taken together, extracellular transglycosylase and GADPH produced by L. plantarum USM8613 could potentially be applied as an alternative therapeutic agent to treat S. aureus skin infections and promote skin health.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Staphylococcus aureus / Glicosiltransferases / Lactobacillus plantarum / Gliceraldeído-3-Fosfato Desidrogenases / Antibacterianos Limite: Humans Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Malásia País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Staphylococcus aureus / Glicosiltransferases / Lactobacillus plantarum / Gliceraldeído-3-Fosfato Desidrogenases / Antibacterianos Limite: Humans Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Malásia País de publicação: Holanda