Development of specific enzyme-linked immunosorbent assays for multiple vitellogenins in marbled sole, Pleuronectes yokohamae.
Gen Comp Endocrinol
; 281: 67-72, 2019 09 15.
Article
em En
| MEDLINE
| ID: mdl-31112714
ABSTRACT
Non-competitive, enzyme-linked immunosorbent assays (ELISAs) for three distinct sole vitellogenins (VtgAa, VtgAb and VtgC) were designed using their purified lipovitellin (Lv) products and corresponding digoxigenin-labeled, anti-Lv polyclonal antibodies, primarily for employment in monitoring estrogenic pollution of the environment. The working range of the ELISAs was from 0.97 to 1,000â¯ng/mL for all Vtg subtypes. Each ELISA appeared to be specific to the targeted Vtg subtype. Intra- and inter-assay coefficients of variation in the developed ELISAs were lower than 10%. Three Vtg subtypes were induced in serum of immature fish by estradiol-17ß (E2) injection (0.5â¯mg/kg body weight). All Vtg subtypes were induced one day after the injection, reaching peak levels (Lv equivalents) within three days, as follows 39.1⯱â¯28.9⯵g/mL (VtgAa), 57.9⯱â¯30.7⯵g/mL (VtgAb) and 12.6⯱â¯4.8⯵g/mL (VtgC). In wild-caught males, VtgAa, VtgAb and VtgC were detected in ranges from 0.26 to 1.21, 0.19 to 8.69, and 0.17 to 53.50⯵g/mL, respectively, over various sampling periods. In vitellogenic females sampled in January, the average level of VtgAb (8,744.43⯱â¯733.93⯵g/mL) was significantly higher than for VtgAa (150.33⯱â¯22.35⯵g/mL) or VtgC (57.08⯱â¯6.00⯵g/mL); thus VtgAb appeared to be the most dominant Vtg subtype. The present study entails the first report on development of subtype-specific Vtg ELISAs in marbled sole, which empowers us to detect and monitor estrogenic contamination in aquatic environments inhabited by this species.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Vitelogeninas
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Peixes
Limite:
Animals
Idioma:
En
Revista:
Gen Comp Endocrinol
Ano de publicação:
2019
Tipo de documento:
Article