Investigating unset endodontic sealers' eugenol and hydrocortisone roles in modulating the initial steps of inflammation.
Clin Oral Investig
; 24(2): 639-647, 2020 Feb.
Article
em En
| MEDLINE
| ID: mdl-31115691
ABSTRACT
INTRODUCTION:
Endodontic treatment success is achieved not only when the cement provides a hermetic seal but also when the injured periapical tissue is regenerated. However, an exaggerated inflammatory reaction hinders tissue regeneration and it has been shown that dental materials affect the inflammatory response through modulation of cytokine secretion. This work was set to investigate the effects of the presence of hydrocortisone in zinc oxide eugenol sealers (Endomethasone N) on modulating the initial steps of inflammation in vitro. MATERIAL ANDMETHODS:
Hydrocortisone and eugenol leaching from Endomethasone N and Pulp Canal Sealer (PCS) were quantified by ELISA and spectrofluorometry, respectively. The effects of Endomethasone N and Pulp Canal Sealer were studied on lipopolysaccharides (LPS)-stimulated human periodontal ligament (hPDL) cells. Cytokine (IL-6, TNF-α) secretion from cells was quantified by ELISA. Inflammatory cell (THP-1) adhesion to activated endothelial cells, their migration and activation were studied in vitro.RESULTS:
Endomethasone N decreased secretion of IL-6 and TNF-α from hPDL cells. THP-1 adhesion to activated endothelial cells (HUVECs) and migration significantly decreased with Endomethasone N while no effect was observed with PCS. Activation of THP-1 decreased with both materials' extracts but was significantly lower with Endomethasone N than with PCS.CONCLUSION:
These results performed in vitro show that Endomethasone N anti-inflammatory effects are due to the presence of hydrocortisone. CLINICAL RELEVANCE Endomethasone N has potential local anti-inflammatory effects which appear to be due to its hydrocortisone rather than eugenol content. Decreasing the inflammatory response is a pre-requisite to initiate the periapical healing.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Materiais Restauradores do Canal Radicular
/
Eugenol
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Hidrocortisona
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Inflamação
Limite:
Humans
Idioma:
En
Revista:
Clin Oral Investig
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
França