P2X7R and PANX-1 channel relevance in a zebrafish larvae copper-induced inflammation model.
Comp Biochem Physiol C Toxicol Pharmacol
; 223: 62-70, 2019 Sep.
Article
em En
| MEDLINE
| ID: mdl-31136852
ABSTRACT
Copper is a metal that participates in several essential reactions in living organisms, and it has been used as an inflammatory inducing agent in zebrafish larvae. In this study, we evaluated the effect P2X7 receptor and/or pannexin channel 1 (PANX-1) blockage in this inflammation model. To perform the experiments, 7 dpf larvae were exposed to 10⯵M of copper and treated with 100⯵M probenecid, PANX-1 inhibitor, and/or 300â¯nM A740003, a P2X7R selective antagonist. Larvae survival was assessed up to 24â¯h after treatments. The evaluation of larvae behavior was evaluated after acute (4â¯h) and chronic (24â¯h) exposure. The parameters of locomotor activity measured were mobile time, average speed, distance and turn angle. We analyzed the gene expression of the P2X7 receptor, PANX1a and PANX1b channels and interleukins IL-10 and IL-1b after 24â¯h of treatment. Treatments did not decrease larval survival in the time interval studied. Changes in larvae locomotion were observed after the longest time of exposure to copper and the treatment with probenecid was able to reverse part of the effects caused by copper. No significant difference was observed in the oxidative stress assays and probenecid and copper treatment decrease partially PANX1a gene expression groups. The data presented herein shows the relevance of the blockage of P2X7-PANX-1 in copper-induced inflammation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
/
Conexinas
/
Cobre
/
Proteínas de Peixe-Zebra
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Receptores Purinérgicos P2X7
/
Inflamação
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Comp Biochem Physiol C Toxicol Pharmacol
Assunto da revista:
FARMACOLOGIA
/
TOXICOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Brasil