Differential promoter utilization by the c-myc gene in mitogen- and interleukin-2-stimulated human lymphocytes.
Mol Cell Biol
; 7(8): 2988-93, 1987 Aug.
Article
em En
| MEDLINE
| ID: mdl-3118189
ABSTRACT
Transcription of the c-myc gene is initiated from two principal promoters, P1 and P2. We demonstrate here that a shift in promoter utilization occurred with time in human peripheral blood mononuclear cells (PBMC) that had been stimulated to proliferate. The P1/P2 ratio reached a maximum of approximately 1.3 at 4 h after phytohemagglutinin stimulation and a minimum of 0.31 at 48 h. Actinomycin decay experiments demonstrated that both P1 and P2 transcripts had similar half-lives at early and late times after mitogen stimulation, indicating that the shift in promoter utilization was probably not posttranscriptionally regulated. Addition of interleukin-2 to previously activated PBMC increased c-myc mRNA, but unlike increases after mitogen stimulation, the P1/P2 ratio stayed less than 0.5. Our findings demonstrated that there was a difference between mitogen- and interleukin-2-stimulated increases in c-myc RNA in PBMC.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proto-Oncogenes
/
Linfócitos
/
Receptores Imunológicos
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Interleucina-2
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Regiões Promotoras Genéticas
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Mitógenos
Limite:
Humans
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1987
Tipo de documento:
Article