mTOR/miR-145-regulated exosomal GOLM1 promotes hepatocellular carcinoma through augmented GSK-3ß/MMPs.
J Genet Genomics
; 46(5): 235-245, 2019 05 20.
Article
em En
| MEDLINE
| ID: mdl-31186161
ABSTRACT
Golgi membrane protein 1 (GOLM1/GP73) is a serum marker of hepatocellular carcinoma (HCC). We have previously shown that mTOR promoted tumorigenesis of HCC through stimulating GOLM1 expression. In this study, we demonstrated that the mammalian target of rapamycin (mTOR) was a negative regulator of microRNA-145 (miR-145) expression. miR-145 inhibited GOLM1 expression by targeting a coding sequence of GOLM1 gene. GOLM1 and miR-145 were inversely correlated in human HCC tissues. GOLM1-enriched exosomes activated the glycogen synthase kinase-3ß/matrix metalloproteinases (GSK-3ß/MMPs) signaling axis of recipient cells and accelerated cell proliferation and migration. In contrast, miR-145 suppressed tumorigenesis and metastasis. We suggest that mTOR/miR-145/GOLM1 signaling pathway should be targeted for HCC treatment.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carcinoma Hepatocelular
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Metaloproteinases da Matriz
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MicroRNAs
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Serina-Treonina Quinases TOR
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Glicogênio Sintase Quinase 3 beta
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Neoplasias Hepáticas
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Proteínas de Membrana
Limite:
Humans
Idioma:
En
Revista:
J Genet Genomics
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China