Eliminating the secondary structure of targeting strands for enhancement of DNA probe based low-abundance point mutation detection.

Ming, Zhihao; Chen, Qianzhi; Chen, Na; Lin, Meng; Liu, Na; Hu, Junbo; Xiao, Xianjin

Ming, Zhihao; Chen, Qianzhi; Chen, Na; Lin, Meng; Liu, Na; Hu, Junbo; Xiao, Xianjin.

Afiliação

Ming Z; Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.
Chen Q; Cancer Research Institute, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, 430030, PR China.
Chen N; Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.
Lin M; Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.
Liu N; Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.
Hu J; Cancer Research Institute, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, 430030, PR China.
Xiao X; Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China. Electronic address: xiaoxianjin@hust.edu.cn.

Anal Chim Acta ; 1075: 137-143, 2019 Oct 10.

Article em En | MEDLINE | ID: mdl-31196419

Assuntos

Sondas de DNA/química; DNA de Cadeia Simples/análise; Proteína BRCA2/genética; Sondas de DNA/genética; DNA de Cadeia Simples/genética; Desoxirribonuclease IV (Fago T4-Induzido)/química; Feminino; Corantes Fluorescentes/química; Humanos; Limite de Detecção; Medições Luminescentes/métodos; Conformação de Ácido Nucleico; Hibridização de Ácido Nucleico; Neoplasias Ovarianas/genética; Mutação Puntual; Telomerase/genética

Palavras-chave

Fluorescent detection; Low-abundance detection; Nucleic acid probes; Point mutations; Secondary structure

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA de Cadeia Simples / Sondas de DNA Tipo de estudo: Diagnostic_studies Limite: Female / Humans Idioma: En Revista: Anal Chim Acta Ano de publicação: 2019 Tipo de documento: Article País de publicação: Holanda

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