Development of fluoroimmunoassays for flecainide.
Ther Drug Monit
; 9(4): 478-83, 1987 Dec.
Article
em En
| MEDLINE
| ID: mdl-3122378
ABSTRACT
Antibodies raised in sheep against a flecainideprotein conjugate and fluorescein-labeled drug were used to develop simple fluoroimmunoassays for the measurement of flecainide acetate in serum or plasma. A rapid, nonseparation assay, based on fluorescence polarization was optimized for therapeutic drug monitoring. A separation fluoroimmunoassay, using antibodies covalently linked to magnetizable particles to avoid the need for centrifugation, was also optimized and validated for monitoring flecainide therapy. It is applicable to lipemic, hemolyzed or icteric samples unsuitable for the nonseparation approach and for laboratories with access only to a simple fluorimeter. Finally, the separation fluoroimmunoassay was modified slightly to improve markedly sensitivity for use in pharmacokinetic studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Flecainida
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Ther Drug Monit
Ano de publicação:
1987
Tipo de documento:
Article
País de afiliação:
Reino Unido