Roundup® confers cytotoxicity through DNA damage and Mitochondria-Associated apoptosis induction.
Environ Pollut
; 252(Pt A): 917-923, 2019 Sep.
Article
em En
| MEDLINE
| ID: mdl-31226516
ABSTRACT
Glyphosate-based herbicides (GBH) are the most widely used pesticides in the world. The extensive use of them increases the potential human health risk, including the human inhalation toxicity risk. We studied the effect of the most famous GBH Roundup® (RDP) in the concentration range from 50 to 125⯵g/mL on Mitochondria-Associated apoptosis and DNA damage in Human alveolar carcinoma cells (A549â¯cells). Alkaline comet assay, immunofluorescence assay and Flow Cytometric Analysis assay were employed to detect DNA damages and apoptosis of A549â¯cells. We found RDP caused concentration-dependent increases in DNA damages and proportion of apoptotic cells in A549â¯cells. RDP induced the DNA single-strand breaks and double-strand breaks; the collapse of mitochondrial membrane by increasing Bax/Bcl-2, resulting in the release of cytochrome c into cytosol and then activated caspase-9/-3, cleaved poly (ADP-ribose) polymerase (PARP) in human lung tissue cells. The results demonstrate that RDP can induce A549â¯cells cytotoxic effects in vitro at the concentration lower than the occupational exposures level of workers, which means RDP has a potential threat to human health.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Glicina
/
Herbicidas
/
Pulmão
/
Mitocôndrias
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Environ Pollut
Assunto da revista:
SAUDE AMBIENTAL
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China