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Molecular recognition of HIV-1 RNAs with branched peptides.
Peralta, Ashley N; Dai, Yumin; Sherpa, Chringma; Le Grice, Stuart F J; Santos, Webster L.
Afiliação
  • Peralta AN; Department of Chemistry and Center for Drug Discovery, Virginia Tech, Blacksburg, VA, United States.
  • Dai Y; Department of Chemistry and Center for Drug Discovery, Virginia Tech, Blacksburg, VA, United States.
  • Sherpa C; Basic Research Laboratory, National Cancer Institute, Frederick, MD, United States.
  • Le Grice SFJ; Basic Research Laboratory, National Cancer Institute, Frederick, MD, United States.
  • Santos WL; Department of Chemistry and Center for Drug Discovery, Virginia Tech, Blacksburg, VA, United States. Electronic address: santosw@vt.edu.
Methods Enzymol ; 623: 373-400, 2019.
Article em En | MEDLINE | ID: mdl-31239054
Targeting RNA offers the potential in many diseases of a therapeutic treatment. Due to its large surface area and ability to adopt different conformations, targeting RNA has proven challenging. Medium-sized branched peptides are of the size to competitively bind RNA while remaining cell permeable, stable in vivo, and non-toxic. Additionally, the ease in generating a large library followed by high-throughput screening provides a way to suggest a scaffold with high diversity that is capable of targeting the structure and sequence of RNA. The ability to select various types of amino acid modifications in the branched peptide allows for variable structures and interactions of the branched peptide but can result in too large a task if not approached properly. In this chapter, we discuss a strategy to selectively recognize RNAs of interest through high throughput screening of branched peptides, validation of hits and biophysical characterization, leading by example with our experience in targeting HIV-1 RNAs with branched peptides.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / RNA Viral / HIV-1 Limite: Humans Idioma: En Revista: Methods Enzymol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / RNA Viral / HIV-1 Limite: Humans Idioma: En Revista: Methods Enzymol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos