The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells.
BMC Res Notes
; 12(1): 370, 2019 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-31262352
ABSTRACT
OBJECTIVE:
Redox homeostasis maintenance is essential to bring about cellular functions. Particularly, embryonic stem cells (ESCs) have high fidelity mechanisms for DNA repair, high activity of different antioxidant enzymes and low levels of oxidative stress. Although the expression and activity of antioxidant enzymes are reduced throughout the differentiation, the knowledge about the transcriptional regulation of genes involved in defense against oxidative stress is yet restricted. Since glutathione is a central component of a complex system involved in preserving cellular redox status, we aimed to study whether the expression of the glutathione reductase (Gsr) gene, which encodes an essential enzyme for cellular redox homeostasis, is modulated by the transcription factors critical for self-renewal and pluripotency of ESCs.RESULTS:
We found that Gsr gene is expressed in ESCs during the pluripotent state and it was upregulated when these cells were induced to differentiate, concomitantly with Nanog decreased expression. Moreover, we found an increase in Gsr mRNA levels when Nanog was downregulated by a specific shRNA targeting this transcription factor in ESCs. Our results suggest that Nanog represses Gsr gene expression in ESCs, evidencing a role of this crucial pluripotency transcription factor in preservation of redox homeostasis in stem cells.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Pluripotentes
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Células-Tronco Embrionárias Murinas
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Proteína Homeobox Nanog
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Glutationa Redutase
Limite:
Animals
Idioma:
En
Revista:
BMC Res Notes
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Argentina