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Sperm Proteome Analysis and Identification of Fertility-Associated Biomarkers in Unexplained Male Infertility.
Panner Selvam, Manesh Kumar; Agarwal, Ashok; Pushparaj, Peter Natesan; Baskaran, Saradha; Bendou, Hocine.
Afiliação
  • Panner Selvam MK; American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA.
  • Agarwal A; American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA. agarwaa@ccf.org.
  • Pushparaj PN; Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Baskaran S; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Bendou H; American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA.
Genes (Basel) ; 10(7)2019 07 11.
Article em En | MEDLINE | ID: mdl-31336797
Up to 30% of men with normal semen parameters suffer from infertility and the reason for this is unknown. Altered expression of sperm proteins may be a major cause of infertility in these men. Proteomic profiling was performed on pooled semen samples from eight normozoospermic fertile men and nine normozoospermic infertile men using LC-MS/MS. Furthermore, key differentially expressed proteins (DEPs) related to the fertilization process were selected for validation using Western blotting. A total of 1139 and 1095 proteins were identified in normozoospermic fertile and infertile men, respectively. Of these, 162 proteins were identified as DEPs. The canonical pathway related to free radical scavenging was enriched with upregulated DEPs in normozoospermic infertile men. The proteins associated with reproductive system development and function, and the ubiquitination pathway were underexpressed in normozoospermic infertile men. Western blot analysis revealed the overexpression of annexin A2 (ANXA2) (2.03 fold change; P = 0.0243), and underexpression of sperm surface protein Sp17 (SPA17) (0.37 fold change; P = 0.0205) and serine protease inhibitor (SERPINA5) (0.32 fold change; P = 0.0073) in men with unexplained male infertility (UMI). The global proteomic profile of normozoospermic infertile men is different from that of normozoospermic fertile men. Our data suggests that SPA17, ANXA2, and SERPINA5 may potentially serve as non-invasive protein biomarkers associated with the fertilization process of the spermatozoa in UMI.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores / Proteínas de Transporte / Anexina A2 / Inibidor da Proteína C / Infertilidade Masculina / Antígenos de Superfície Tipo de estudo: Diagnostic_studies / Etiology_studies / Risk_factors_studies Limite: Humans / Male Idioma: En Revista: Genes (Basel) Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores / Proteínas de Transporte / Anexina A2 / Inibidor da Proteína C / Infertilidade Masculina / Antígenos de Superfície Tipo de estudo: Diagnostic_studies / Etiology_studies / Risk_factors_studies Limite: Humans / Male Idioma: En Revista: Genes (Basel) Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Suíça