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Inhibition of Axon Regeneration by Liquid-like TIAR-2 Granules.
Andrusiak, Matthew G; Sharifnia, Panid; Lyu, Xiaohui; Wang, Zhiping; Dickey, Andrea M; Wu, Zilu; Chisholm, Andrew D; Jin, Yishi.
Afiliação
  • Andrusiak MG; Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Sharifnia P; Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Lyu X; Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Wang Z; Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Dickey AM; Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Wu Z; Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Chisholm AD; Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Jin Y; Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: yijin@ucsd.edu.
Neuron ; 104(2): 290-304.e8, 2019 10 23.
Article em En | MEDLINE | ID: mdl-31378567
Phase separation into liquid-like compartments is an emerging property of proteins containing prion-like domains (PrLDs), yet the in vivo roles of phase separation remain poorly understood. TIA proteins contain a C-terminal PrLD, and mutations in the PrLD are associated with several diseases. Here, we show that the C. elegans TIAR-2/TIA protein functions cell autonomously to inhibit axon regeneration. TIAR-2 undergoes liquid-liquid phase separation in vitro and forms granules with liquid-like properties in vivo. Axon injury induces a transient increase in TIAR-2 granule number. The PrLD is necessary and sufficient for granule formation and inhibiting regeneration. Tyrosine residues within the PrLD are important for granule formation and inhibition of regeneration. TIAR-2 is also serine phosphorylated in vivo. Non-phosphorylatable TIAR-2 variants do not form granules and are unable to inhibit axon regeneration. Our data demonstrate an in vivo function for phase-separated TIAR-2 and identify features critical for its function in axon regeneration.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Axônios / Proteínas de Caenorhabditis elegans / Proteínas com Motivo de Reconhecimento de RNA / Regeneração Nervosa Limite: Animals Idioma: En Revista: Neuron Assunto da revista: NEUROLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Axônios / Proteínas de Caenorhabditis elegans / Proteínas com Motivo de Reconhecimento de RNA / Regeneração Nervosa Limite: Animals Idioma: En Revista: Neuron Assunto da revista: NEUROLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos