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An overview of decellularisation techniques of native tissues and tissue engineered products for bone, ligament and tendon regeneration.
Blaudez, F; Ivanovski, S; Hamlet, S; Vaquette, C.
Afiliação
  • Blaudez F; Griffith University, School of Dentistry, Gold Coast, Australia.
  • Ivanovski S; The University of Queensland, School of Dentistry, Herston, Queensland, Australia.
  • Hamlet S; Griffith University, School of Dentistry, Gold Coast, Australia.
  • Vaquette C; The University of Queensland, School of Dentistry, Herston, Queensland, Australia. Electronic address: c.vaquette@uq.edu.au.
Methods ; 171: 28-40, 2020 01 15.
Article em En | MEDLINE | ID: mdl-31394166
Decellularised tissues and organs have been successfully used in a variety of tissue engineering/regenerative medicine applications. Because of the complexity of each tissue (size, porosity, extracellular matrix (ECM) composition etc.), there is no standardised protocol and the decellularisation methods vary widely, thus leading to heterogeneous outcomes. Physical, chemical, and enzymatic methods have been developed and optimised for each specific application and this review describes the most common strategies utilised to achieve decellularisation of soft and hard tissues. While removal of the DNA is the primary goal of decellularisation, it is generally achieved at the expense of ECM preservation due to the harsh chemical or enzymatic processing conditions. As denaturation of the native ECM has been associated with undesired host responses, decellularisation conditions aimed at effectively achieving simultaneous DNA removal and minimal ECM damage will be highlighted. Additionally, the utilisation of decellularised matrices in regenerative medicine is explored, as are the most recent strategies implemented to circumvent challenges in this field. In summary, this review focusses on the latest advancements and future perspectives in the utilisation of natural ECM for the decoration of synthetic porous scaffolds.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regeneração Óssea / Engenharia Tecidual / Matriz Extracelular / Alicerces Teciduais Limite: Animals / Humans Idioma: En Revista: Methods Assunto da revista: BIOQUIMICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Austrália País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regeneração Óssea / Engenharia Tecidual / Matriz Extracelular / Alicerces Teciduais Limite: Animals / Humans Idioma: En Revista: Methods Assunto da revista: BIOQUIMICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Austrália País de publicação: Estados Unidos