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RNAi as a Tool to Study Virulence in the Pathogenic Yeast Candida glabrata.
Ishchuk, Olena P; Ahmad, Khadija Mohamed; Koruza, Katarina; Bojanovic, Klara; Sprenger, Marcel; Kasper, Lydia; Brunke, Sascha; Hube, Bernhard; Säll, Torbjörn; Hellmark, Thomas; Gullstrand, Birgitta; Brion, Christian; Freel, Kelle; Schacherer, Joseph; Regenberg, Birgitte; Knecht, Wolfgang; Piskur, Jure.
Afiliação
  • Ishchuk OP; Department of Biology, Lund University, Lund, Sweden.
  • Ahmad KM; Department of Biology and Biological Engineering, Systems and Synthetic Biology, Chalmers University of Technology, Gothenburg, Sweden.
  • Koruza K; Department of Biology, Lund University, Lund, Sweden.
  • Bojanovic K; Department of Biology, Lund University, Lund, Sweden.
  • Sprenger M; Department of Biology, Lund University, Lund, Sweden.
  • Kasper L; Department of Microbial Pathogenicity Mechanisms, Hans Knöll Institute, Jena, Germany.
  • Brunke S; Department of Microbial Pathogenicity Mechanisms, Hans Knöll Institute, Jena, Germany.
  • Hube B; Department of Microbial Pathogenicity Mechanisms, Hans Knöll Institute, Jena, Germany.
  • Säll T; Department of Microbial Pathogenicity Mechanisms, Hans Knöll Institute, Jena, Germany.
  • Hellmark T; Institute of Microbiology, Friedrich Schiller University, Jena, Germany.
  • Gullstrand B; Department of Biology, Lund University, Lund, Sweden.
  • Brion C; Department of Nephrology, Lund University, Lund, Sweden.
  • Freel K; Department of Nephrology, Lund University, Lund, Sweden.
  • Schacherer J; Department of Molecular Genetics, Genomics and Microbiology, Strasbourg University, Strasbourg, France.
  • Regenberg B; Department of Molecular Genetics, Genomics and Microbiology, Strasbourg University, Strasbourg, France.
  • Knecht W; Department of Molecular Genetics, Genomics and Microbiology, Strasbourg University, Strasbourg, France.
  • Piskur J; Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen, Denmark.
Front Microbiol ; 10: 1679, 2019.
Article em En | MEDLINE | ID: mdl-31396189
The yeast Candida glabrata is a major opportunistic pathogen causing mucosal and systemic infections in humans. Systemic infections caused by this yeast have high mortality rates and are difficult to treat due to this yeast's intrinsic and frequently adapting antifungal resistance. To understand and treat C. glabrata infections, it is essential to investigate the molecular basis of C. glabrata virulence and resistance. We established an RNA interference (RNAi) system in C. glabrata by expressing the Dicer and Argonaute genes from Saccharomyces castellii (a budding yeast with natural RNAi). Our experiments with reporter genes and putative virulence genes showed that the introduction of RNAi resulted in 30 and 70% gene-knockdown for the construct-types antisense and hairpin, respectively. The resulting C. glabrata RNAi strain was used for the screening of a gene library for new virulence-related genes. Phenotypic profiling with a high-resolution quantification of growth identified genes involved in the maintenance of cell integrity, antifungal drugs, and ROS resistance. The genes identified by this approach are promising targets for the treatment of C. glabrata infections.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Front Microbiol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Suécia País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Front Microbiol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Suécia País de publicação: Suíça