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P Granules Protect RNA Interference Genes from Silencing by piRNAs.
Ouyang, John Paul T; Folkmann, Andrew; Bernard, Lauren; Lee, Chih-Yung; Seroussi, Uri; Charlesworth, Amanda G; Claycomb, Julie M; Seydoux, Geraldine.
Afiliação
  • Ouyang JPT; HHMI and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
  • Folkmann A; HHMI and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
  • Bernard L; HHMI and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
  • Lee CY; HHMI and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
  • Seroussi U; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
  • Charlesworth AG; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
  • Claycomb JM; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
  • Seydoux G; HHMI and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: gseydoux@jhmi.edu.
Dev Cell ; 50(6): 716-728.e6, 2019 09 23.
Article em En | MEDLINE | ID: mdl-31402283
P granules are perinuclear condensates in C. elegans germ cells proposed to serve as hubs for self/non-self RNA discrimination by Argonautes. We report that a mutant (meg-3 meg-4) that does not assemble P granules in primordial germ cells loses competence for RNA-interference over several generations and accumulates silencing small RNAs against hundreds of endogenous genes, including the RNA-interference genes rde-11 and sid-1. In wild type, rde-11 and sid-1 transcripts are heavily targeted by piRNAs and accumulate in P granules but maintain expression. In the primordial germ cells of meg-3 meg-4 mutants, rde-11 and sid-1 transcripts disperse in the cytoplasm with the small RNA biogenesis machinery, become hyper-targeted by secondary sRNAs, and are eventually silenced. Silencing requires the PIWI-class Argonaute PRG-1 and the nuclear Argonaute HRDE-1 that maintains trans-generational silencing of piRNA targets. These observations support a "safe harbor" model for P granules in protecting germline transcripts from piRNA-initiated silencing.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Caenorhabditis elegans / Grânulos Citoplasmáticos / RNA Interferente Pequeno / Interferência de RNA Limite: Animals Idioma: En Revista: Dev Cell Assunto da revista: EMBRIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Caenorhabditis elegans / Grânulos Citoplasmáticos / RNA Interferente Pequeno / Interferência de RNA Limite: Animals Idioma: En Revista: Dev Cell Assunto da revista: EMBRIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos