Antioxidant compounds extracted from Diaporthe schini using supercritical CO2 plus cosolvent.
Bioprocess Biosyst Eng
; 43(1): 133-141, 2020 Jan.
Article
em En
| MEDLINE
| ID: mdl-31542822
ABSTRACT
Endophytic fungi have been highlight in the production of secondary metabolites with different bioactive properties, such as in the production of the antioxidant compounds. Therefore, the objective of this work was the extraction of the antioxidant compounds from the biomass of Diaporthe schini using supercritical carbon dioxide (CO2) without and with ethanol as cosolvent. The biomass was produced by submerged fermentation and the parameters evaluated in the extraction process were pressure (150-250 bar), temperature (40-60 ºC) and cosolvent [biomass cosolvent ratio, 10, 10.75 and 11.5 (w/v)]. Extraction yield, antioxidant activity and chemical composition of the extracts were determined. The highest extraction yield (3.24 wt.%) and the best antioxidant activity against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (96.62%) were obtained at 40 ºC, 250 bar and biomasscosolvent ratio of 11.5 (w/v). The chemical compounds 1,4-diaza-2,5-dioxo-3-isobutyl bicyclo[4.3.0]nonane and benzeneethanol identified in GC/MS could be responsible for the antioxidant activity found in this study.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ascomicetos
/
Dióxido de Carbono
/
Etanol
/
Antioxidantes
Idioma:
En
Revista:
Bioprocess Biosyst Eng
Assunto da revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY