The use of carotenoids and oxonol VI as probes for membrane potential in proteoliposomes.
FEBS Lett
; 184(1): 96-9, 1985 May 06.
Article
em En
| MEDLINE
| ID: mdl-3157600
ABSTRACT
Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelength is obtained with valinomycin-induced potassium ion diffusion potentials, irrespective of the polarity of the potassium gradient. In contrast to this, the (externally added) probe oxonol VI only shows an absorbance band shift when the external potassium ion concentration is higher than the internal one. In liposomes without ATPase complex, no carotenoid absorbance band shifts were observed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxazóis
/
Proteolipídeos
/
Carotenoides
/
Isoxazóis
/
Potenciais da Membrana
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1985
Tipo de documento:
Article