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Mitsui-7, heat-treated, and nitrogen-doped multi-walled carbon nanotubes elicit genotoxicity in human lung epithelial cells.
Siegrist, Katelyn J; Reynolds, Steven H; Porter, Dale W; Mercer, Robert R; Bauer, Alison K; Lowry, David; Cena, Lorenzo; Stueckle, Todd A; Kashon, Michael L; Wiley, John; Salisbury, Jeffrey L; Mastovich, John; Bunker, Kristin; Sparrow, Mark; Lupoi, Jason S; Stefaniak, Aleksandr B; Keane, Michael J; Tsuruoka, Shuji; Terrones, Mauricio; McCawley, Michael; Sargent, Linda M.
Afiliação
  • Siegrist KJ; Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Rd, Morgantown, WV, 26505, USA.
  • Reynolds SH; Department of Occupational and Environmental Health Sciences, West Virginia University, Morgantown, WV, 26506, USA.
  • Porter DW; Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Rd, Morgantown, WV, 26505, USA.
  • Mercer RR; Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Rd, Morgantown, WV, 26505, USA.
  • Bauer AK; Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Rd, Morgantown, WV, 26505, USA.
  • Lowry D; Anschutz Medical Campus, Department of Environmental and Occupational Health, University of Colorado, Aurora, CO, 80045, USA.
  • Cena L; Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Rd, Morgantown, WV, 26505, USA.
  • Stueckle TA; Department of Health, West Chester University, West Chester, PA, 19383, USA.
  • Kashon ML; Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Rd, Morgantown, WV, 26505, USA.
  • Wiley J; Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Rd, Morgantown, WV, 26505, USA.
  • Salisbury JL; Department of Pediatrics, East Carolina University, Greenville, NC, 27834, USA.
  • Mastovich J; Mayo Clinic, Rochester, MN, 55905, USA.
  • Bunker K; Bruker AXS Inc, Madison, WI, 53711, USA.
  • Sparrow M; RJ Lee Group, 350 Hochberg Road, Monroeville, PA, 15146, USA.
  • Lupoi JS; Independent Consultant, Allison Park, PA, 15101, USA.
  • Stefaniak AB; RJ Lee Group, 350 Hochberg Road, Monroeville, PA, 15146, USA.
  • Keane MJ; Respiratory Health Division, National Institute for Occupational Safety and Health, Morgantown, WV, 26505, USA.
  • Tsuruoka S; Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Rd, Morgantown, WV, 26505, USA.
  • Terrones M; Shinshu University, Nagano City, Japan.
  • McCawley M; Pennsylvania State University, State College, PA, 16801, USA.
  • Sargent LM; Department of Occupational and Environmental Health Sciences, West Virginia University, Morgantown, WV, 26506, USA.
Part Fibre Toxicol ; 16(1): 36, 2019 10 07.
Article em En | MEDLINE | ID: mdl-31590690
BACKGROUND: The unique physicochemical properties of multi-walled carbon nanotubes (MWCNT) have led to many industrial applications. Due to their low density and small size, MWCNT are easily aerosolized in the workplace making respiratory exposures likely in workers. The International Agency for Research on Cancer designated the pristine Mitsui-7 MWCNT (MWCNT-7) as a Group 2B carcinogen, but there was insufficient data to classify all other MWCNT. Previously, MWCNT exposed to high temperature (MWCNT-HT) or synthesized with nitrogen (MWCNT-ND) have been found to elicit attenuated toxicity; however, their genotoxic and carcinogenic potential are not known. Our aim was to measure the genotoxicity of MWCNT-7 compared to these two physicochemically-altered MWCNTs in human lung epithelial cells (BEAS-2B & SAEC). RESULTS: Dose-dependent partitioning of individual nanotubes in the cell nuclei was observed for each MWCNT material and was greatest for MWCNT-7. Exposure to each MWCNT led to significantly increased mitotic aberrations with multi- and monopolar spindle morphologies and fragmented centrosomes. Quantitative analysis of the spindle pole demonstrated significantly increased centrosome fragmentation from 0.024-2.4 µg/mL of each MWCNT. Significant aneuploidy was measured in a dose-response from each MWCNT-7, HT, and ND; the highest dose of 24 µg/mL produced 67, 61, and 55%, respectively. Chromosome analysis demonstrated significantly increased centromere fragmentation and translocations from each MWCNT at each dose. Following 24 h of exposure to MWCNT-7, ND and/or HT in BEAS-2B a significant arrest in the G1/S phase in the cell cycle occurred, whereas the MWCNT-ND also induced a G2 arrest. Primary SAEC exposed for 24 h to each MWCNT elicited a significantly greater arrest in the G1 and G2 phases. However, SAEC arrested in the G1/S phase after 72 h of exposure. Lastly, a significant increase in clonal growth was observed one month after exposure to 0.024 µg/mL MWCNT-HT & ND. CONCLUSIONS: Although MWCNT-HT & ND cause a lower incidence of genotoxicity, all three MWCNTs cause the same type of mitotic and chromosomal disruptions. Chromosomal fragmentation and translocations have not been observed with other nanomaterials. Because in vitro genotoxicity is correlated with in vivo genotoxic response, these studies in primary human lung cells may predict the genotoxic potency in exposed human populations.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dano ao DNA / Nanotubos de Carbono / Células Epiteliais / Temperatura Alta / Pulmão / Nitrogênio Limite: Humans Idioma: En Revista: Part Fibre Toxicol Assunto da revista: TOXICOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dano ao DNA / Nanotubos de Carbono / Células Epiteliais / Temperatura Alta / Pulmão / Nitrogênio Limite: Humans Idioma: En Revista: Part Fibre Toxicol Assunto da revista: TOXICOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido