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Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation.
Wang, Meitian; Xu, Zhiliang; Liu, Qiao; Sun, Wenjie; Jiang, Baichun; Yang, Kun; Li, Jiangxia; Gong, Yaoqin; Liu, Qiji; Liu, Duo; Li, Xi.
Afiliação
  • Wang M; Key Laboratory of Experimental Teratology, Ministry of Education Department of Medical Genetics, School of Basic Medical Sciences, Shandong University Jinan, Shandong, 250012, China.
  • Xu Z; Key Laboratory of Experimental Teratology, Ministry of Education Department of Medical Genetics, School of Basic Medical Sciences, Shandong University Jinan, Shandong, 250012, China.
  • Liu Q; Key Laboratory of Experimental Teratology, Ministry of Education Department of Medical Genetics, School of Basic Medical Sciences, Shandong University Jinan, Shandong, 250012, China.
  • Sun W; Key Laboratory of Experimental Teratology, Ministry of Education Department of Medical Genetics, School of Basic Medical Sciences, Shandong University Jinan, Shandong, 250012, China.
  • Jiang B; Key Laboratory of Experimental Teratology, Ministry of Education Department of Medical Genetics, School of Basic Medical Sciences, Shandong University Jinan, Shandong, 250012, China.
  • Yang K; Key Laboratory of Experimental Teratology, Ministry of Education Department of Medical Genetics, School of Basic Medical Sciences, Shandong University Jinan, Shandong, 250012, China.
  • Li J; Key Laboratory of Experimental Teratology, Ministry of Education Department of Medical Genetics, School of Basic Medical Sciences, Shandong University Jinan, Shandong, 250012, China.
  • Gong Y; Key Laboratory of Experimental Teratology, Ministry of Education Department of Medical Genetics, School of Basic Medical Sciences, Shandong University Jinan, Shandong, 250012, China.
  • Liu Q; Key Laboratory of Experimental Teratology, Ministry of Education Department of Medical Genetics, School of Basic Medical Sciences, Shandong University Jinan, Shandong, 250012, China.
  • Liu D; State Key Laboratory of Crystal Materials, Shandong University Jinan, Shandong, 250100, PR China.
  • Li X; Key Laboratory of Experimental Teratology, Ministry of Education Department of Medical Genetics, School of Basic Medical Sciences, Shandong University Jinan, Shandong, 250012, China; Advanced Medical Research Institute, Shandong University Jinan, Shandong, 250012, China. Electronic address: lixi@sdu
Biomaterials ; 225: 119539, 2019 12.
Article em En | MEDLINE | ID: mdl-31622821
ABSTRACT
Photostimulation has been widely used in neuromodulation. However, existing optogenetics techniques require genetic alternation of the targeted cell or tissue. Here, we report that neural stem cells (NSCs) constitutionally express blue/red light-sensitive photoreceptors. The proliferation and regulation of NSCs to neuronal or glial cells are wavelength-specific. Our results showed a 4.3-fold increase in proliferation and 2.7-fold increase in astrocyte differentiation for cells under low-power blue monochromatic light exposure (455 nm, 300 µW/cm2). The melanopsin (Opn4)/transient receptor potential channel 6 (TRPC6) non-visual opsin serves as a key photoreceptor response to blue light irradiation. Two-dimensional gel electrophoresis coupled with mass spectrometry further highlighted the Jun activation domain-binding protein 1 (Jab1) as a novel and specific modulator in phototransduction pathways induced by blue light exposure. Quiescent adult NSCs reside in specific regions of the mammalian brain. Therefore, we showed that melanopsin/TRPC6 expressed in these regions and blue light stimulation through optical fibers could directly stimulate the NSCs in vivo. Upconversion nanoparticles (UCNPs) converted deep-penetrating near-infrared (NIR) light into specific wavelengths of visible light. Accordingly, we demonstrated that UCNP-mediated NIR light could be used to modulate in vivo NSC differentiation in a less invasive manner. In the future, this light-triggered system of NSCs will enable nongenetic and noninvasive neuromodulation with therapeutic potential for central nervous system diseases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Neuroglia / Células-Tronco Neurais / Optogenética / Neurônios Limite: Animals Idioma: En Revista: Biomaterials Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Neuroglia / Células-Tronco Neurais / Optogenética / Neurônios Limite: Animals Idioma: En Revista: Biomaterials Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China