Nitrogen-doped carbon dots-V2O5 nanobelts sensing platform for sensitive detection of ascorbic acid and alkaline phosphatase activity.
Anal Chim Acta
; 1089: 131-143, 2019 Dec 16.
Article
em En
| MEDLINE
| ID: mdl-31627810
ABSTRACT
In this work, the as-prepared V2O5 nanobelts can sensitively quench the fluorescence of nitrogen-doped carbon dots (N-CDs) based on the inner filter effect (IFE). In the presence of ascorbic acid (AA), the fluorescence of N-CDs can recover through the redox reaction between V2O5 nanobelts and AA. Meanwhile, in the presence of both alkaline phosphatase (ALP) and ascorbyl-2-phosphate (AAP), the fluorescence of N-CDs can also restore since AAP can be hydrolyzed into AA by ALP. Under optimum conditions, the linear range for AA is from 0.01 to 2.5⯵M with a detection limit of 3â¯nM and that for ALP is from 0.1 to 30 U/L with a detection limit of 0.04 U/L (S/Nâ¯=â¯3). Particularly, the proposed probe could be successfully used to detect AA and ALP in human serum samples. Furthermore, N-CDs can be applied in fluorescence imaging of Human breast cancer cells with satisfactory results.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Ascórbico
/
Compostos de Vanádio
/
Pontos Quânticos
/
Fosfatase Alcalina
/
Corantes Fluorescentes
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China