HPLC-ESI- MS/MS analysis of beet (Beta vulgaris) leaves and its beneficial properties in type 1 diabetic rats.
Biomed Pharmacother
; 120: 109541, 2019 Dec.
Article
em En
| MEDLINE
| ID: mdl-31629949
ABSTRACT
The phenolic profile of the leaves of Beta vulgaris subspecies vulgaris variety rubra was investigated by high-performance liquid chromatography (HPLC) coupled to electrospray ionization high resolution mass spectrometric (ESI-HRMS-MS) detection. Mass spectrometry-based molecular networking was employed to dereplicate the known compounds. Twelve known compounds, seven of which are previously undescribed as constituents in the B. vulgaris leaves were dereplicated and assigned with various levels of identification confidence. The ameliorative effects of the aqueous methanolic extract of the leaves were assessed against alloxan induced diabetic rats. It was found that the extract significantly decreased (p < 0.001) serum glucose, lipid profile, ALT, AST, TNF-α, IL-1ß, IL-6, and hepatic MDA levels; and significantly increased (pâ¯<â¯0.001) hepatic TAO and GSH; and down-regulated the expression of hepatic NF-κB versus the untreated diabetic groups, in a dose-dependent manner. In molecular docking, all identified compounds exhibited good glide score against the PPAR-É£ target, confirming the in vivo observed activities. In conclusion, B. vulgaris has immunomodulatory / antioxidant effects that could be helpful in slowing the progression of diabetic complications.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
/
Folhas de Planta
/
Espectrometria de Massas por Ionização por Electrospray
/
Beta vulgaris
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Diabetes Mellitus Experimental
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Diabetes Mellitus Tipo 1
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Espectrometria de Massas em Tandem
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
/
Male
Idioma:
En
Revista:
Biomed Pharmacother
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Egito
País de publicação:
FR
/
FRANCE
/
FRANCIA
/
FRANÇA