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Overexpression of Limb-Bud and Heart (LBH) promotes angiogenesis in human glioma via VEGFA-mediated ERK signalling under hypoxia.
Jiang, Yang; Zhou, Jinpeng; Zou, Dan; Hou, Dianqi; Zhang, Haiying; Zhao, Junshuang; Li, Long; Hu, Jiangfeng; Zhang, Ye; Jing, Zhitao.
Afiliação
  • Jiang Y; Department of Neurosurgery, the First Hospital of China Medical University, No. 155, North Nanjing Street, Shenyang, Liaoning 110001, China; Department of Neurosurgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 100 Haining Road, Shanghai 200080, China.
  • Zhou J; Department of Neurosurgery, the First Hospital of China Medical University, No. 155, North Nanjing Street, Shenyang, Liaoning 110001, China.
  • Zou D; The First Laboratory of Cancer Institute, the First Hospital of China Medical University, No. 155, North Nanjing Street, Shenyang, Liaoning 110001, China.
  • Hou D; Department of Neurosurgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 100 Haining Road, Shanghai 200080, China.
  • Zhang H; International Education College, Liaoning University of Traditional Chinese Medicine, No. 79 Chongshan East Road, Shenyang, Liaoning 110042, China.
  • Zhao J; Department of Neurosurgery, the First Hospital of China Medical University, No. 155, North Nanjing Street, Shenyang, Liaoning 110001, China.
  • Li L; Department of Neurosurgery, the First Hospital of China Medical University, No. 155, North Nanjing Street, Shenyang, Liaoning 110001, China.
  • Hu J; Department of Gastroenterology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 100 Haining Road, Shanghai 200080, China.
  • Zhang Y; The First Laboratory of Cancer Institute, the First Hospital of China Medical University, No. 155, North Nanjing Street, Shenyang, Liaoning 110001, China. Electronic address: yzhang21@cmu.edu.cn.
  • Jing Z; Department of Neurosurgery, the First Hospital of China Medical University, No. 155, North Nanjing Street, Shenyang, Liaoning 110001, China. Electronic address: jingzhitao@hotmail.com.
EBioMedicine ; 48: 36-48, 2019 Oct.
Article em En | MEDLINE | ID: mdl-31631037
ABSTRACT

BACKGROUND:

Glioma is the most common primary malignant tumor in the central nervous system with frequent hypoxia and angiogenesis. Limb-Bud and Heart (LBH) is a highly conserved transcription cofactor that participates in embryonic development and tumorigenesis.

METHODS:

The conditioned media from LBH regulated human glioma cell lines and patient-derived glioma stem cells (GSCs) were used to treat the human brain microvessel endothelial cells (hBMECs). The function of LBH on angiogenesis were examined through methods of MTS assay, Edu assay, TUNEL assay, western blotting analysis, qPCR analysis, luciferase reporter assay and xenograft experiment.

FINDINGS:

Our study found for the first time that LBH was overexpressed in gliomas and was associated with a poor prognosis. LBH overexpression participated in the angiogenesis of gliomas via the vascular endothelial growth factor A (VEGFA)-mediated extracellular signal-regulated kinase (ERK) signalling pathway in human brain microvessel endothelial cells (hBMECs). Rapid proliferation of gliomas can lead to tissue hypoxia and hypoxia inducible factor-1 (HIF-1) activation, while HIF-1 can directly transcriptionally regulate the expression of LBH and result in a self-reinforcing cycle.

INTERPRETATION:

LBH may be a possible treatment target to break the vicious cycle in glioma treatment.    .
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Fator A de Crescimento do Endotélio Vascular / MAP Quinases Reguladas por Sinal Extracelular / Glioma / Neovascularização Patológica Tipo de estudo: Prognostic_studies Limite: Animals / Female / Humans Idioma: En Revista: EBioMedicine Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Fator A de Crescimento do Endotélio Vascular / MAP Quinases Reguladas por Sinal Extracelular / Glioma / Neovascularização Patológica Tipo de estudo: Prognostic_studies Limite: Animals / Female / Humans Idioma: En Revista: EBioMedicine Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China