Effect of ethanol extracts of Hericium erinaceus mycelium on morphineinduced microglial migration.
Mol Med Rep
; 20(6): 5279-5285, 2019 Dec.
Article
em En
| MEDLINE
| ID: mdl-31638202
ABSTRACT
Microglia serve important roles in chronic pain signal transduction pathways. Glia cells, especially microglia, seem to share mechanisms that lead to chronic pain and morphineinduced tolerance. Evidence has suggested that downregulating cytoskeleton activity in microglia provides pain relief in chronic pain and morphine tolerance. The purpose of the present study was to evaluate the effect of ethanol extracts of Hericium erinaceus (EHE) mycelium on morphineinduced BV2 microglial cell activation. BV2 cells were starved for 4 h in DMEM before being incubated with 100 ng/ml EHE for 30 min, followed by 1 µM morphine for 2 h. Subsequently, the cells were harvested and used for migration experiments and western blotting. The results showed that 1 µM morphine enhanced BV2 cell activation and chemotactic reaction, and it increased histone deacetylase 6 (HDAC6) expression and heat shock protein 90 (HSP90) deacetylation as well as HSP90 cleavage. Pretreatment with 100 ng/ml EHE significantly inhibited the morphinestimulated effects on BV2 cells. The present study demonstrated that EHE inhibited morphineinduced BV2 activations by regulating the HDAC6/HSP90 deacetylation signal transduction pathway.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Basidiomycota
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Movimento Celular
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Microglia
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Micélio
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Misturas Complexas
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Morfina
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2019
Tipo de documento:
Article