Characterization of the gut microbiota in frail elderly patients.
Aging Clin Exp Res
; 32(10): 2001-2011, 2020 Oct.
Article
em En
| MEDLINE
| ID: mdl-31656031
ABSTRACT
BACKGROUND:
The change in the composition of gut microbiota has been reported in the elderly and in the frail individuals; however, studies on gut microbiota in frail elderly are limited.AIMS:
This study aimed to investigate the gut microbiota of the frail elderly.METHODS:
From September 2017 to February 2018, 27 elderly patients hospitalized in the Department of Geriatrics of our hospital were enrolled and divided into the frailty group (n = 15) and the control group (n = 12) based on the cutoff of 0.25 for the frailty index. The fecal samples were collected for 16S rRNA-amplicon sequencing to analyze the composition and richness of gut microbiota. Operational taxonomic unit (OTU) clustering was performed using Usearch software. Intra-sample diversity (alpha-diversity) analysis and inter-sample diversity (beta-diversity) analyses were performed. The community richness was compared between the two groups at family and genus levels.RESULTS:
There were 1903 and 1880 OTUs identified in the control and frailty groups, respectively, with 1282 OTUs overlap between the two groups. The alpha diversity of microbiota community was similar between the two groups, whereas the frailty group had larger beta diversity than the control group. The top-10 taxonomy categories and abundances of gut microbiota between the two groups were similar. As for the gut microbiota composition, 4 families and 17 genera were significantly different between the two groups (p < 0.05).CONCLUSION:
These results suggested that frailty can affect gut microbiota diversity and compositions in late elderly hospitalized patients.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microbioma Gastrointestinal
/
Fragilidade
Limite:
Aged
/
Humans
Idioma:
En
Revista:
Aging Clin Exp Res
Assunto da revista:
GERIATRIA
Ano de publicação:
2020
Tipo de documento:
Article