Potential utility of metagenomic sequencing for improving etiologic diagnosis of infective endocarditis.
Future Cardiol
; 15(6): 411-424, 2019 11.
Article
em En
| MEDLINE
| ID: mdl-31691592
ABSTRACT
Aim:
To explore potential utility of metagenomic sequencing for improving etiologic diagnosis of infective endocarditis (IE) caused by fastidious bacteria. Materials &methods:
Plasma and heart valves of two patients, who were diagnosed with IE caused by Bartonella quintana and Propionibacterium species, were sequenced by using Illumina MiSeq and Nanopore MinION.Results:
For patient 1, B. quintana was detected in the plasma pool collected 4 days before valvular replacement surgery. For patient 2, Propionibacterium sp. oral taxon 193 was detected in the plasma sample collected on hospital day 1. Nearly complete bacterial genomes (>98%) were retrieved from resected heart valves of both patients, enabling detection of antibiotic resistance-associated features. Real-time sequencing of heart valves identified both pathogens within the first 16 min of sequencing runs.Conclusion:
Metagenomic sequencing may be a helpful supplement to IE diagnostic workflow, especially when conventional tests fail to yield a diagnosis.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
DNA Bacteriano
/
Endocardite Bacteriana
/
Metagenômica
/
Valvas Cardíacas
Tipo de estudo:
Diagnostic_studies
/
Etiology_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Future Cardiol
Assunto da revista:
CARDIOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article