Labeling Acidic Compartments of Neutrophils with Cresyl Violet.
Methods Mol Biol
; 2087: 207-213, 2020.
Article
em En
| MEDLINE
| ID: mdl-31728993
ABSTRACT
We introduce the acidotropic marker cresyl violet to stain acidic granules in live neutrophils. Cresyl violet is less phototoxic, more photostable, and more cost-effective than other commercially available acidotropic markers. Additionally, it does not photoconvert to fluorescent species of a different color, a limitation of other commonly used acidotropic markers. Staining can be readily detected by fluorescence microscopy or by flow cytometry, and can be used as a readout of degranulation in activated neutrophils.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
/
Benzoxazinas
/
Corantes Fluorescentes
/
Microscopia de Fluorescência
/
Neutrófilos
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Canadá