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microRNA-501-5p promotes cell proliferation and migration in gastric cancer by downregulating LPAR1.
Ma, Xiang; Feng, Jiaxi; Lu, Ming; Tang, Wenjuan; Han, Jianbo; Luo, XiaGang; Zhao, Qinghong; Yang, Li.
Afiliação
  • Ma X; Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
  • Feng J; Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
  • Lu M; Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
  • Tang W; Department of Newborn Infants, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
  • Han J; Department of General Surgery, Nanjing Red Cross Hospital, Nanjing, Jiangsu, China.
  • Luo X; Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
  • Zhao Q; Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
  • Yang L; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
J Cell Biochem ; 121(2): 1911-1922, 2020 02.
Article em En | MEDLINE | ID: mdl-31746031
ABSTRACT
In spite of the achievement in treatment, the gastric cancer (GC) mortality still remains high. MicroRNAs (miRNAs) are a group of small noncoding RNAs that play a crucial part in tumor progression. In this study, we explored the expression and function of microRNA-501-5p (miR-501-5p) in GC cell lines. Quantitative real-time polymerase chain reaction assay results suggested that miR-501-5p was significantly upregulated in GC tissues and cell lines. And, the Cell Counting Kit-8 colony formation and cell migration assay results showed that the downregulation of miR-501-5p decreased GC cell proliferation and migration. Besides that, we found that GC cell cycle was arrested in G2 phase and cell apoptosis rate was increased by silencing the expression of miR-501-5p in GC cell lines using the flow cytometry. We also found that miR-501-5p could directly target lysophosphatidic acid receptor 1 (LPAR1) and negatively regulate LPAR1 expression in GC cell lines by performing dual-luciferase reporter gene assay and Western blot analysis. And, LPAR1 was significantly downregulated in GC tissues and inversely correlated with miR-501-5p expression. Furthermore, LPAR1 downregulation promoted cell proliferation and migration, which were attenuated by cotransfection of miR-501-5p inhibitor in GC cells. In conclusion, miR-501-5p can promote GC cell proliferation and migration by targeting and downregulating LPAR1. miR-501-5p/LPAR1 may become a potential therapeutic target for GC treatment.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Biomarcadores Tumorais / Regulação Neoplásica da Expressão Gênica / Movimento Celular / MicroRNAs / Receptores de Ácidos Lisofosfatídicos / Proliferação de Células Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Cell Biochem Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Biomarcadores Tumorais / Regulação Neoplásica da Expressão Gênica / Movimento Celular / MicroRNAs / Receptores de Ácidos Lisofosfatídicos / Proliferação de Células Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Cell Biochem Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China