LncRNA ANCR downregulates hypoxiainducible factor 1α and inhibits the growth of HPVnegative cervical squamous cell carcinoma under hypoxic conditions.
Mol Med Rep
; 21(1): 413-419, 2020 01.
Article
em En
| MEDLINE
| ID: mdl-31746351
ABSTRACT
Long noncoding RNA (lncRNA) antidifferentiation noncoding RNA (ANCR) has been reported to participate in numerous types of malignancies. The present study aimed to investigate the function of lncRNA ANCR in cervical squamous cell carcinoma (CSCC). The expression of ANCR in the cervical tissues (tumor tissues in patients with CSCC) and serum of patients with CSCC in addition to healthy female controls was detected using reverse transcriptionquantitative polymerase chain reaction. Diagnostic values of ANCR expression in cervical tissue and serum for CSCC were determined using receiver operating characteristic curve analysis. LncRNA ANCR and hypoxiainducible factor 1α (HIF1α) expression vectors were constructed and transfected into CSCC cell lines, and cell proliferation under normal O2 and hypoxic conditions (8% O2) was detected using a Cell Counting kit8 assay. Expression of HIF1α was determined using western blot analysis. It was observed that ANCR was downregulated in human papillomavirus (HPV)negative patients with CSCC compared with in normal female cases and HPVpositive patients with CSCC in cervical tissues and in the serum, and the downregulation of ANCR effectively distinguished HPVnegative patients with CSCC from healthy controls. ANCR overexpression inhibited the proliferation of HPVnegative CSCC cells under hypoxic conditions, whilst HIF1α overexpression reversed this effect. ANCR overexpression inhibited HIF1α expression in HPVnegative CSCC cells, while HIF1α overexpression exhibited no significant effect on ANCR expression. It was therefore concluded that ANCR may inhibit the growth of HPVnegative cervical squamous cell carcinoma under hypoxic conditions by downregulating HIF1α.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carcinoma de Células Escamosas
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Neoplasias do Colo do Útero
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Subunidade alfa do Fator 1 Induzível por Hipóxia
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RNA Longo não Codificante
Limite:
Adult
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Female
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Humans
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Middle aged
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2020
Tipo de documento:
Article