Bioassay-guided purification, GC-MS characterization and quantification of phyto-components in an antibacterial extract of Searsia lancea leaves.
Nat Prod Res
; 35(22): 4658-4662, 2021 Nov.
Article
em En
| MEDLINE
| ID: mdl-31820661
ABSTRACT
Phytocompounds in an aqueous methanol (70% MeOH) leaf extract of Searsia lancea were separated using liquid-liquid partitioning techniques and gravity-assisted column chromatography. The resultant fractions were screened for antibacterial properties (minimum inhibitory concentration, MIC) against four bacterial strains (Enterococcus faecalis, Klebsiella pneumoniae, Neisseria gonorrhoeae and Staphylococcus aureus). Bioactive fractions were purified using preparative thin layer chromatography (TLC) and subjected to further antibacterial screening. Phytocompounds in antibacterial sub-fractions were characterized and quantified using Gas Chromatography-Mass Spectrometry (GC-MS). An ethyl acetate sub-fraction purified from the aqueous methanol extracts of the leaves demonstrated potent antibacterial properties (MIC range 31-61 µg/ml against E. faecalis and S. aureus). Based on GC-MS analysis, 81.5% of the sub-fraction consisted of broad-spectrum antibacterial compounds namely tetracosanol (43.98%) and nonadecanol (37.5%). Current research findings support the traditional use of S. lancea leaves to manage gastro-intestinal disorders and gonorrhoea.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Staphylococcus aureus
/
Extratos Vegetais
Idioma:
En
Revista:
Nat Prod Res
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
África do Sul