One step multi-material 3D printing for the fabrication of a photometric detector flow cell.
Anal Chim Acta
; 1097: 127-134, 2020 Feb 08.
Article
em En
| MEDLINE
| ID: mdl-31910952
ABSTRACT
Optical detection is the most common detection mode for many analytical assays. Photometric detection systems and their integration with analytical systems usually require several assembly parts and manual alignment of the capillary/tubing which affects sensitivity and repeatability. 3D printing is an innovative technology for the fabrication of integrated complex detection systems. One step multi-material 3D printing has been explored to fabricate a photometric detector flow cell from optically transparent and opaque materials using a dual-head FDM 3D printer. Integration of the microchannel, the detection window and the slit in a single device eliminates the need for manual alignment of fluidic and optical components, and hence improves sensitivity and repeatability. 3D printing allowed for rapid design optimisation by varying the slit dimension and optical pathlength. The optimised design was evaluated by determining stray light, effective path length and the signal to noise ratio using orange G. The optimised flow cell with extended path length of 10â¯mm and 500⯵m slit yielded 0.02% stray light, 89% effective path length and detection limit of 2â¯nM. The sensitivity was also improved by 80% in the process of optimisation, using a blue 470â¯nm LED as a light source.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Austrália