Localization, in human placenta, of the tightly bound form of DNA methylase in the higher order of chromatin organization.
Biochim Biophys Acta
; 951(1): 191-200, 1988 Nov 10.
Article
em En
| MEDLINE
| ID: mdl-3191132
ABSTRACT
In human placenta, the DNA of all subfractions of the third level of chromatin organization exhibits similar values of the methylcytosine-to-cytosine ratio. The tightly bound form of DNA methyltransferase is mostly recovered in the 'stripped loop' fraction, although, on the basis of the DNA content, the 'stripped loops' and the 'stripped matrix' appear to possess a similar amount of the enzyme. DNA methyltransferase activity is instead totally absent from the 'digested matrix', i.e., from the fraction remaining after digestion of the 'stripped matrix' with DNAase I. Upon addition of exogenous DNA methyltransferase, however, the DNA of this fraction, which is only 1% (in weight) of the total chromatin DNA and which has a length of approx. 9 kbp, can readily undergo methylation.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Placenta
/
Cromatina
/
DNA (Citosina-5-)-Metiltransferases
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
Itália