Simultaneous determination of fluoxetine, venlafaxine, vortioxetine and their active metabolites in human plasma by LC-MS/MS using one-step sample preparation procedure.
J Pharm Biomed Anal
; 181: 113098, 2020 Mar 20.
Article
em En
| MEDLINE
| ID: mdl-31978643
The aim of antidepressant therapy is to induce remission and prevent relapses of major depressive disorder with minimum adverse effects during the treatment. Due to high variability in metabolism, therapeutic drug monitoring is recommended as a useful tool for individualisation of the therapy. For this purpose, we have developed simple and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for quantification of fluoxetine (FLX), venlafaxine (VEN), vortioxetine (VTX) and their active metabolites norfluoxetine (NFLX) and O-desmethylvenlafaxine (ODV). After one-step extraction procedure using OSTRO plate, analytes were separated by gradient elution on Acquity UPLC BEH C18 (50â¯×â¯2.1â¯mm, 1.7⯵m) column with runtime 4.2â¯min. The detection was done on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode with transitions at m/z 310.23 â 148.20 for FLX, m/z 296.23 â 134.20 for NFLX, m/z 278.31 â 121.13 for VEN, m/z 264.31 â 107.14 for ODV and m/z 299.19 â 150.05 for VTX using a positive electrospray ionisation interface. The method was successfully validated according to the European Medicine Agency guideline for the selectivity, linearity and lower limit of detection, precision and accuracy, matrix effect, extraction recovery, carryover, dilution integrity and stability over a concentration range of 1-300â¯ng/mL for FLX, NFLX, VEN, ODV and 0.2-100â¯ng/mL VTX. Extraction recovery for each analyte was > 80 %, and no significant matrix effects were observed. The developed method was employed for quantification of antidepressants in clinical samples from patients treated with either FLX, VEN, or VTX.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fluoxetina
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Extração Líquido-Líquido
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Cloridrato de Venlafaxina
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Vortioxetina
Tipo de estudo:
Guideline
Limite:
Adolescent
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Adult
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Aged
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Child
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Humans
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Middle aged
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2020
Tipo de documento:
Article
País de publicação:
Reino Unido