Correlation between detergent activity and anti-herpes simplex virus-2 activity of commercially available vaginal gels.
BMC Res Notes
; 13(1): 52, 2020 Jan 31.
Article
em En
| MEDLINE
| ID: mdl-32005126
ABSTRACT
OBJECTIVE:
Herpes simplex virus-2 (HSV-2) infections are almost exclusively sexually transmitted. The presence of vaginal gels during sexual activity may have a significant positive or negative impact on viral transmission. Therefore we investigated three off-the-shelf vaginal lubricants and one pH restoring gel to evaluate their impact on HSV-2 replication.RESULTS:
HeLa cells were infected with untreated virions and virions incubated with the particular gels. The accumulation of viral genomes was monitored by quantitative PCR (qPCR) method at 24 h post infection. Two of the tested gels had no significant effect on HSV-2 replication at the maximum applied concentration, while two had a strong inhibitory effect (~ 98% reduction of replication). The replication inhibitory effect was observed at various multiplicity of infection (MOI 0.4-6.4) and the two inhibitory gels were also capable of inhibiting the HSV-2 induced cytopathic effect on HeLa cells. The surface tension decreasing activity-an indication of detergent activity-was strongly correlated with the anti-HSV-2 activity of the gels (R2 0.88). Our results indicate that off-the-shelf vaginal gels have a markedly different anti-HSV-2 activity that may influence HSV-2 transmission.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antivirais
/
Cremes, Espumas e Géis Vaginais
/
Herpesvirus Humano 2
/
Detergentes
Limite:
Humans
Idioma:
En
Revista:
BMC Res Notes
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Hungria