Engineering of Cyclodextrin Glycosyltransferase Reveals pH-Regulated Mechanism of Enhanced Long-Chain Glycosylated Sophoricoside Specificity.

Han, Ruizhi; Ni, Jie; Zhou, Jieyu; Dong, Jinjun; Xu, Guochao; Ni, Ye

Han, Ruizhi; Ni, Jie; Zhou, Jieyu; Dong, Jinjun; Xu, Guochao; Ni, Ye.

Afiliação

Han R; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Ni J; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Zhou J; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Dong J; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Xu G; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Ni Y; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.

Appl Environ Microbiol ; 86(7)2020 03 18.

Article em En | MEDLINE | ID: mdl-32005733

Assuntos

Proteínas de Bactérias/genética; Benzopiranos/metabolismo; Glucosiltransferases/genética; Paenibacillus/genética; Polissacarídeos/metabolismo; Proteínas de Bactérias/metabolismo; Glucosiltransferases/metabolismo; Glicosilação; Concentração de Íons de Hidrogênio; Cinética; Paenibacillus/enzimologia; Paenibacillus/metabolismo; Engenharia de Proteínas; Especificidade por Substrato

Palavras-chave

cyclodextrin glycosyltransferase; long-chain glycosylated product specificity; molecular dynamics; saturation mutagenesis; sophoricoside glycosylation

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polissacarídeos / Proteínas de Bactérias / Benzopiranos / Paenibacillus / Glucosiltransferases Idioma: En Revista: Appl Environ Microbiol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos

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