A comparative study of extraction techniques for maximum recovery of ß-galactosidase from the yogurt bacterium Lactobacillus delbrueckii ssp. bulgaricus.
J Dairy Res
; 87(1): 123-126, 2020 Feb.
Article
em En
| MEDLINE
| ID: mdl-32008592
ABSTRACT
The study reported in this research communication evaluates the chemical (solvents) and mechanical (sonication, bead-beater) extraction methods to determine the maximum recovery of ß-galactosidase from L. bulgaricus spp. Among all extraction techniques, sonication-assisted extraction yielded the highest amounts of enzyme activity (between 1892-2156 Miller Units) in cell-free extract (supernatant). Interestingly, solvent extracted enzyme activities were found to be very low (between 83-153 Miller Units) in supernatant. SDS-polyacrylamide gel electrophoresis and the total protein determination showed that mechanical methods can completely lyse the cells. Our results thus demonstrated that the mechanical extraction method of sonication is the best one for recovering the maximum amount of lactase from L. bulgaricus strains.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Iogurte
/
Beta-Galactosidase
/
Lactobacillus delbrueckii
Idioma:
En
Revista:
J Dairy Res
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos