Tissue transglutaminase_variant 2-transduced mesenchymal stem cells and their chondrogenic potential.
Biotechnol Bioeng
; 117(6): 1839-1852, 2020 06.
Article
em En
| MEDLINE
| ID: mdl-32068240
ABSTRACT
As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) in mesenchymal stem cells (MSC) derived from bone marrow of rats. For this purpose, rat MSCs transduced with TGM2_v2 were grown and differentiated on three-dimensional polybutylene succinate (PBSu) and poly-l-lactide (PLLA) blend scaffolds. The transduced cells could not only successfully express the short form transglutaminase-2, but also deposited the protein onto the scaffolds. In addition, they could spontaneously produce cartilage-specific proteins without any chondrogenic induction, suggesting that TGM2_v2 expression provided the cells the ability of chondrogenic differentiation. PBSuPLLA scaffolds loaded with TGM2_v2 expressing MSCs could be used in repair of articular cartilage defects.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução Genética
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Transglutaminases
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Proteínas de Ligação ao GTP
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Condrogênese
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Células-Tronco Mesenquimais
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Biotechnol Bioeng
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Turquia