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A Direct Fluorescent Activity Assay for Glycosyltransferases Enables Convenient High-Throughput Screening: Application to O-GlcNAc Transferase.
Alteen, Matthew G; Gros, Christina; Meek, Richard W; Cardoso, David A; Busmann, Jil A; Sangouard, Gontran; Deen, Matthew C; Tan, Hong-Yee; Shen, David L; Russell, Cecilia C; Davies, Gideon J; Robinson, Phillip J; McCluskey, Adam; Vocadlo, David J.
Afiliação
  • Alteen MG; Department of Chemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
  • Gros C; Department of Chemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
  • Meek RW; York Structural Biology Laboratory, Department of Chemistry, University of York, York, YO10 5DD, UK.
  • Cardoso DA; Children's Medical Research Institute, The University of Sydney, Sydney, NSW, 2145, Australia.
  • Busmann JA; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
  • Sangouard G; Department of Chemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
  • Deen MC; Department of Chemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
  • Tan HY; Department of Chemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
  • Shen DL; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
  • Russell CC; Chemistry, School of Environmental and Life Sciences, The University of Newcastle, University Drive, Callaghan, NSW, 2308, Australia.
  • Davies GJ; York Structural Biology Laboratory, Department of Chemistry, University of York, York, YO10 5DD, UK.
  • Robinson PJ; Children's Medical Research Institute, The University of Sydney, Sydney, NSW, 2145, Australia.
  • McCluskey A; Chemistry, School of Environmental and Life Sciences, The University of Newcastle, University Drive, Callaghan, NSW, 2308, Australia.
  • Vocadlo DJ; Department of Chemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
Angew Chem Int Ed Engl ; 59(24): 9601-9609, 2020 06 08.
Article em En | MEDLINE | ID: mdl-32092778

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Fluorescência / N-Acetilglucosaminiltransferases / Ensaios Enzimáticos Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Revista: Angew Chem Int Ed Engl Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Canadá

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Fluorescência / N-Acetilglucosaminiltransferases / Ensaios Enzimáticos Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Revista: Angew Chem Int Ed Engl Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Canadá