Skin Microcolumns as a Source of Paracrine Signaling Factors.
Adv Wound Care (New Rochelle)
; 9(4): 174-183, 2020 04 01.
Article
em En
| MEDLINE
| ID: mdl-32117581
ABSTRACT
Objective:
We recently developed the approach of using "microcolumns" of autologous full-thickness skin tissue for wound repair. The small size of these micro skin tissue columns (MSTCs, â¼0.5 mm in diameter) allows donor sites to heal quickly without scarring. Treatment with MSTCs significantly accelerate wound healing, and suppled various skin cell types and skin structures to replenish the wound volume. This technology is now starting clinical use. In this study, we investigate whether MSTCs may also influence wound healing by releasing soluble signaling factors.Approach:
Freshly harvested MSTCs were incubated in culture medium for 24 h. The conditioned medium was collected and tested for its effects on migration and proliferation of human dermal fibroblasts, and its ability to induce tube formation by human umbilical vein endothelial cells (HUVECs). Proteins released into the conditioned medium were characterized by multiplex enzyme-linked immunosorbent assay (ELISA), and compared with medium conditioned by an equivalent mass of intact full-thickness skin.Results:
MSTC-conditioned medium increased fibroblast migration and proliferation, as well as HUVEC tube formation. MSTCs released many soluble factors known to play prominent roles in wound healing. A subset of proteins showed significantly different release profiles compared with intact full-thickness skin. Innovation The technology for harvesting and using MSTCs to augment wound healing was recently developed as an alternative to conventional autologous skin grafting. This study shows that MSTCs could also function as "cytokine factories."Conclusion:
In addition to supplying autologous cells to repopulate the wound volume, MSTCs can also function as a source of growth factors and cytokines to further enhance wound healing.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Cicatrização
/
Citocinas
/
Transplante de Pele
/
Comunicação Parácrina
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Fibroblastos
Limite:
Humans
Idioma:
En
Revista:
Adv Wound Care (New Rochelle)
Ano de publicação:
2020
Tipo de documento:
Article