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Circ_0000388 Exerts Oncogenic Function in Cervical Cancer Cells by Regulating miR-337-3p/TCF12 Axis.
Meng, Qing-Hua; Li, Ying; Kong, Cui; Gao, Xue-Mei; Jiang, Xiao-Juan.
Afiliação
  • Meng QH; Department of Gynaecology and Obstetrics, Linyi Central Hospital, Linyi, China.
  • Li Y; Department of Gynaecology and Obstetrics, The Third People's Hospital of Linyi, Linyi, China.
  • Kong C; Department of Gynaecology and Obstetrics, The Third People's Hospital of Linyi, Linyi, China.
  • Gao XM; Department of Gynaecology and Obstetrics, Linyi Central Hospital, Linyi, China.
  • Jiang XJ; Department of Gynaecology and Obstetrics, Jinan Maternal and Child Health Hospital of Shandong Province, Jinan, China.
Cancer Biother Radiopharm ; 36(1): 58-69, 2021 Feb.
Article em En | MEDLINE | ID: mdl-32119786
Background: Cervical cancer (CC) is one of the most common cancers among women worldwide. Circular RNAs (circRNAs) are recently identified as important gene regulators with critical roles in cancer biology. In this study, we explored the effects of circ_0000388 on the malignant phenotypes of CC cells and its mechanism. Materials and Methods: Circ_0000388 expression and miR-337-3p expression in CC tissue samples were measured using quantitative real time polymerase chain reaction. CCK-8 was adopted to assess the effect of circ_0000388 on CC cell line proliferation. TUNEL assay was employed to probe the effect of circ_0000388 on apoptosis. Wound healing assay and transwell assay were conducted to detect the effect of circ_0000388 on migration and invasion. Further, interaction among circ_0000388, miR-337-3p, and TCF12 (transcription factor 12) was determined by bioinformatics analysis, RT-PCR, Western blot, RNA immunoprecipitation assay, and luciferase reporter assay. Results: Circ_0000388 expression in CC clinical samples was upregulated and this was correlated with unfavorable pathological indexes. Circ_0000388 remarkably enhanced the proliferation and metastasis of CC cells. Circ_0000388 overexpression dramatically impeded miR-337-3p expression and it was identified as a sponge of miR-337-3p. Furthermore, circ_00003888 also enhanced the TCF12 expression, while the effect could be reversed by co-transfection with miR-377-3p. Conclusions: Circ_0000388 was a novel oncogenic circRNA in CC, and promoted cancer progression via regulating miR-337-3p and TCF12, and could be potentially used as a diagnostic biomarker and therapy target.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias do Colo do Útero / MicroRNAs / Fatores de Transcrição Hélice-Alça-Hélice Básicos / Carcinogênese / RNA Circular Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: Cancer Biother Radiopharm Assunto da revista: FARMACIA / FARMACOLOGIA / NEOPLASIAS / TERAPEUTICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias do Colo do Útero / MicroRNAs / Fatores de Transcrição Hélice-Alça-Hélice Básicos / Carcinogênese / RNA Circular Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: Cancer Biother Radiopharm Assunto da revista: FARMACIA / FARMACOLOGIA / NEOPLASIAS / TERAPEUTICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos