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Quantification and characterization of granulocyte macrophage colony-stimulating factor activated human peripheral blood mononuclear cells by fluorine-19 cellular MRI in an immunocompromised mouse model.
Fink, C; Smith, M; Sehl, O C; Gaudet, J M; Meagher, T C; Sheikh, N A; Dikeakos, J D; Rieder, M J; Foster, P J; Dekaban, G A.
Afiliação
  • Fink C; Biotherapeutics Research Laboratory, Robarts Research Institute, London, Ontario N6A 5B7, Canada; Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5B7, Canada.
  • Smith M; Biotherapeutics Research Laboratory, Robarts Research Institute, London, Ontario N6A 5B7, Canada; Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5B7, Canada.
  • Sehl OC; Imaging Research Laboratories, Robarts Research Institute, London, Ontario N6A 5B7, Canada; Department of Medical Biophysics, University of Western Ontario, London, Ontario N6A 5B7, Canada.
  • Gaudet JM; Imaging Research Laboratories, Robarts Research Institute, London, Ontario N6A 5B7, Canada; Department of Medical Biophysics, University of Western Ontario, London, Ontario N6A 5B7, Canada.
  • Meagher TC; Dendreon Corporation, Seattle, Washington 98102, USA.
  • Sheikh NA; Dendreon Corporation, Seattle, Washington 98102, USA.
  • Dikeakos JD; Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5B7, Canada.
  • Rieder MJ; Biotherapeutics Research Laboratory, Robarts Research Institute, London, Ontario N6A 5B7, Canada; Department of Pediatrics, University of Western Ontario, London, Ontario N6A 5B7, Canada.
  • Foster PJ; Imaging Research Laboratories, Robarts Research Institute, London, Ontario N6A 5B7, Canada; Department of Medical Biophysics, University of Western Ontario, London, Ontario N6A 5B7, Canada.
  • Dekaban GA; Biotherapeutics Research Laboratory, Robarts Research Institute, London, Ontario N6A 5B7, Canada; Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5B7, Canada. Electronic address: dekaban@robarts.ca.
Diagn Interv Imaging ; 101(9): 577-588, 2020 Sep.
Article em En | MEDLINE | ID: mdl-32122802
ABSTRACT

PURPOSE:

The purpose of this study was to test fluorine-19 (19F) cellular magnetic resonance (MRI) as a non-invasive imaging modality to track therapeutic cell migration as a surrogate marker of immunotherapeutic effectiveness. MATERIALS AND

METHODS:

Human peripheral blood mononuclear cell- (PBMC)-derived antigen presenting cell (APC) were labeled with a 19F-perfluorocarbon (PFC) and/or activated with granulocyte macrophage colony-stimulating factor (GM-CSF). Viability, phenotype and cell lineage characterization preceded 19F cellular MRI of PFC+ PBMC under both pre-clinical 9.4 Tesla (T) and clinical 3T conditions in a mouse model.

RESULTS:

A high proportion of PBMC incorporated PFC without affecting viability, phenotype or cell lineage composition. PFC+ PBMC were in vivo migration-competent to draining and downstream lymph nodes. GM-CSF addition to culture increased PBMC migration to, and persistence within, secondary lymphoid organs.

CONCLUSION:

19F cellular MRI is a non-invasive imaging technique capable of detecting and quantifying in vivo cell migration in conjunction with an established APC-based immunotherapy model. 19F cellular MRI can function as a surrogate marker for assessing and improving upon the therapeutic benefit that this immunotherapy provides.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Fator Estimulador de Colônias de Granulócitos e Macrófagos Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Diagn Interv Imaging Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Canadá
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Fator Estimulador de Colônias de Granulócitos e Macrófagos Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Diagn Interv Imaging Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Canadá