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Fecal gluten immunogenic peptides as indicators of dietary compliance in celiac patients.
Porcelli, Brunetta; Ferretti, Fabio; Cinci, Francesca; Biviano, Ivano; Santini, Alessia; Grande, Elisabetta; Quagliarella, Francesco; Terzuoli, Lucia; Bacarelli, Maria R; Bizzaro, Nicola; Vascotto, Marina; Marini, Mario.
Afiliação
  • Porcelli B; Section of Biochemistry, Department of Medical Biotechnologies, University of Siena, Siena, Italy - brunetta.porcelli@unisi.it.
  • Ferretti F; Department of Medicine, Surgery and Neuroscience, University of Siena, Siena, Italy.
  • Cinci F; Section of Biochemistry, Department of Medical Biotechnologies, University of Siena, Siena, Italy.
  • Biviano I; Unit of Gastroenterology and Operative Endoscopy, Siena University Hospital, Siena, Italy.
  • Santini A; Unit of Gastroenterology and Operative Endoscopy, Siena University Hospital, Siena, Italy.
  • Grande E; Unit of Pediatrics, Siena University Hospital, Siena, Italy.
  • Quagliarella F; Unit of Pediatrics, Siena University Hospital, Siena, Italy.
  • Terzuoli L; Section of Biochemistry, Department of Medical Biotechnologies, University of Siena, Siena, Italy.
  • Bacarelli MR; Department of Medicine, Surgery and Neuroscience, University of Siena, Siena, Italy.
  • Bizzaro N; Laboratory of Clinical Pathology, San Antonio Hospital Tolmezzo, Udine Integrated University Healthcare Company, Udine, Italy.
  • Vascotto M; Unit of Pediatrics, Siena University Hospital, Siena, Italy.
  • Marini M; Unit of Gastroenterology and Operative Endoscopy, Siena University Hospital, Siena, Italy.
Minerva Gastroenterol Dietol ; 66(3): 201-207, 2020 Sep.
Article em En | MEDLINE | ID: mdl-32218420
ABSTRACT

BACKGROUND:

It is important to have methods for evaluating dietary compliance in patients with celiac disease (CD). Determination of fecal gluten immunogenic peptides (GIPs) was recently proposed as a method of detecting gluten intake. The aim of this study was to evaluate whether determination of GIPs can be used as an indicator of compliance with a gluten-free diet (GFD).

METHODS:

Twenty-five persons with CD on a gluten-free diet for at least one year were enrolled in the study. Compliance with the diet was assessed by the Biagi questionnaire, evaluation of symptoms and assay of IgA anti-tissue transglutaminase antibodies (IgA anti-tTG). GIPs were determined by iVYLISA GIP-S test (Biomedal S.L., Seville, Spain) on an automated Chorus analyzer (DIESSE Diagnostica Senese, Siena, Italy), after extraction of fecal samples by the method developed by DIESSE.

RESULTS:

Four patients tested positive for GIPs (GIP+), two of whom complied strictly with the gluten-free diet according to the Biagi questionnaire. None of the four GIP-positive patients manifested symptoms. IgA anti-tTG was significantly higher in GIP+ than in GIP- subjects.

CONCLUSIONS:

Assay of fecal GIPs identified more patients who were not complying with the diet than the Biagi questionnaire or evaluation of symptoms. The anti-tTG and GIP results agreed perfectly; however, since anti-tTG antibodies remain high for longer and are not a completely reliable marker of GFD intake, detection of fecal GIPs offers a direct, objective, quantitative assessment of exposure, even occasional, to gluten and could be used to check dietary compliance.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença Celíaca / Cooperação do Paciente / Dieta Livre de Glúten / Fezes / Glutens Limite: Adolescent / Adult / Aged / Child / Female / Humans / Male / Middle aged Idioma: En Revista: Minerva Gastroenterol Dietol Assunto da revista: CIENCIAS DA NUTRICAO / GASTROENTEROLOGIA Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença Celíaca / Cooperação do Paciente / Dieta Livre de Glúten / Fezes / Glutens Limite: Adolescent / Adult / Aged / Child / Female / Humans / Male / Middle aged Idioma: En Revista: Minerva Gastroenterol Dietol Assunto da revista: CIENCIAS DA NUTRICAO / GASTROENTEROLOGIA Ano de publicação: 2020 Tipo de documento: Article